FACSCalibur Cell Analyzer

2-laser, 4 color (3-1)

Consumables

 (1)  5-mL polystyrene test tubes, 12 x 75-mm (BD Falcon Cat. No.352052)

1. Sample preparation

(1) Recommend sample concentration: 1x10⁶ cells /ml

(2) Filter your samples

Recommend filtering samples through a nylon mesh 40 µm (BD Cat. No. 352340) or 5-mL polystyrene test tubes, 12 x 75-mm (BD Falcon) with cell-strainer cap (BD Cat. No. 352235) if aggregated cells can be seen in the sample tube.

Filtered sample should be saved on ice, protected from light.

Data

Immunophenotyping

Characterizing cells at different stages of development through the use of fluorescent-labeled monoclonal antibodies against surface markers is one of the most common applications of flow cytometry. The BD FACSCalibur system provides multicolor capability in an easy-to-use system. Instrument setup is as easy as the push of a button and a click of the mouse. Efficient collection optics, coupled to the flow cell with an optical gel, permit the use of a low-powered, air-cooled argon laser and a red diode laser to ensure the high sensitivity required for analysis of low-density antigens.

DNA analysis

The BD FACSCalibur system works together with BD DNA analysis quality control products to eliminate many of the variables associated with ploidy and cell-cycle analysis. The instrument’s optical and fluidic design ensures that high-resolution DNA ploidy and S-phase determinations are easy to achieve. Electronic doublet discrimination, using the area and width of the fluorescence pulse, allows for the removal of artifacts—doublets and aggregates—from the analysis. High-resolution electronics and linearity ensure accurate results. To ensure optimal performance, BD DNA QC products, including fixed cells and fluorescent particles, can be used for setup, verification of the doublet discrimination module, and monitoring daily operation.

Attachment:

   1. BD_FACSCalibur_instructions

   2. CellQuest prouser guide

   3. FACSCalibur quickguide

BD Bioscience http://www.bdbiosciences.com/tw/instruments/facscalibur/features/applications.jsp